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rabbit polyclonal anti tgf beta receptor ii antibody  (Bioss)


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    Bioss rabbit polyclonal anti tgf beta receptor ii antibody
    Rabbit Polyclonal Anti Tgf Beta Receptor Ii Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+tgf+beta+receptor+ii+antibody/TGF+beta+Receptor+II+Polyclonal+Antibody/pmc08185144-80-27-34
    Average 90 stars, based on 2 article reviews
    rabbit polyclonal anti tgf beta receptor ii antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: Integrating miRNA and mRNA Profiling to Assess the Potential miRNA–mRNA Modules Linked With Testicular Immune Homeostasis in Sheep
    Article Snippet: .. In brief, sections were blocked with 5% bovine serum albumin (BSA) for 1 h and incubated with rabbit polyclonal anti-CD19 antibody (Bioss, Beijing, China; 1:150 dilution) or rabbit polyclonal anti-TGF beta receptor II antibody (Bioss, Beijing, China; 1:200 dilution). ..

    Article Title: Integrating miRNA and mRNA Profiling to Assess the Potential miRNA-mRNA Modules Linked With Testicular Immune Homeostasis in Sheep.
    Article Snippet: .. Membranes were incubated with rabbit polyclonal anti-CD19 antibody (Bioss, Beijing, China; 1:500 dilution), rabbit polyclonal anti-TGF beta receptor II antibody (Bioss, Beijing, China; 1:500 dilution), or rabbit polyclonal anti-beta-actin antibody (Bioss, Beijing, China; 1:1,500 dilution) and the secondary antibody goat anti-rabbit IgG conjugated with HRP (Bioss, Beijing, China; 1:5,000 dilution). .. Protein bands were visualized using an ECL kit (NCM Biotech, Suzhou, China) and quantified using AlphaEaseFC software (Protein Simple, Santa Clara, CA, USA).

    Article Title: Integrating miRNA and mRNA Profiling to Assess the Potential miRNA-mRNA Modules Linked With Testicular Immune Homeostasis in Sheep.
    Article Snippet: .. In brief, sections were blocked with 5% bovine serum albumin (BSA) for 1 h and incubated with rabbit polyclonal anti-CD19 antibody (Bioss, Beijing, China; 1:150 dilution) or rabbit polyclonal anti-TGF beta receptor II antibody (Bioss, Beijing, China; 1:200 dilution). ..

    Article Title: Integrating miRNA and mRNA Profiling to Assess the Potential miRNA–mRNA Modules Linked With Testicular Immune Homeostasis in Sheep
    Article Snippet: .. Membranes were incubated with rabbit polyclonal anti-CD19 antibody (Bioss, Beijing, China; 1:500 dilution), rabbit polyclonal anti-TGF beta receptor II antibody (Bioss, Beijing, China; 1:500 dilution), or rabbit polyclonal anti-beta-actin antibody (Bioss, Beijing, China; 1:1,500 dilution) and the secondary antibody goat anti-rabbit IgG conjugated with HRP (Bioss, Beijing, China; 1:5,000 dilution). .. Protein bands were visualized using an ECL kit (NCM Biotech, Suzhou, China) and quantified using AlphaEaseFC software (Protein Simple, Santa Clara, CA, USA).



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    rabbit polyclonal anti tgf beta receptor ii antibody  (Bioss)
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    Combination of pirfenidone and SB525334 altered the expression of NK cells’ receptors in mice with CE. (A) Mice were inoculated with 5000 protoscoleces, and pirfenidone and SB525334 were administered one month after infection. Samples were collected after one month of medication. (B) Only the pirfenidone and combination medication groups showing decreased secretion of <t>TGF-β</t> in CE liver tissues. (C) and (D) Compared to the other groups, serum ALT and AST levels are the lowest in the combined medication category. Similar results are evident with the levels of serum DBL. (E) Only SB525334 can reduce the secretion of DBL in the plasma of CE mice. (F) The three treatments elevate the expression of NK cell surface receptors DX5 and NKG2D and reduce the expression of NK cell surface receptor TIM3. Both SB525334 and combined medication can reduce the expression of the NKG2A receptor on the surface of NK cells, but pirfenidone cannot. (G) Proliferative index Ki67of NK cells was an evident increase in the combined drug group compared to both the individual drugs and control groups. Data are representative of one of three independent experiments (n = 3–4 mice per group). One-way ANOVA was used for analysis in (B)–(F), bars represent the mean ± s.e.m. (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001).
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    Combination of pirfenidone and SB525334 altered the expression of NK cells’ receptors in mice with CE. (A) Mice were inoculated with 5000 protoscoleces, and pirfenidone and SB525334 were administered one month after infection. Samples were collected after one month of medication. (B) Only the pirfenidone and combination medication groups showing decreased secretion of <t>TGF-β</t> in CE liver tissues. (C) and (D) Compared to the other groups, serum ALT and AST levels are the lowest in the combined medication category. Similar results are evident with the levels of serum DBL. (E) Only SB525334 can reduce the secretion of DBL in the plasma of CE mice. (F) The three treatments elevate the expression of NK cell surface receptors DX5 and NKG2D and reduce the expression of NK cell surface receptor TIM3. Both SB525334 and combined medication can reduce the expression of the NKG2A receptor on the surface of NK cells, but pirfenidone cannot. (G) Proliferative index Ki67of NK cells was an evident increase in the combined drug group compared to both the individual drugs and control groups. Data are representative of one of three independent experiments (n = 3–4 mice per group). One-way ANOVA was used for analysis in (B)–(F), bars represent the mean ± s.e.m. (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001).
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    Combination of pirfenidone and SB525334 altered the expression of NK cells’ receptors in mice with CE. (A) Mice were inoculated with 5000 protoscoleces, and pirfenidone and SB525334 were administered one month after infection. Samples were collected after one month of medication. (B) Only the pirfenidone and combination medication groups showing decreased secretion of <t>TGF-β</t> in CE liver tissues. (C) and (D) Compared to the other groups, serum ALT and AST levels are the lowest in the combined medication category. Similar results are evident with the levels of serum DBL. (E) Only SB525334 can reduce the secretion of DBL in the plasma of CE mice. (F) The three treatments elevate the expression of NK cell surface receptors DX5 and NKG2D and reduce the expression of NK cell surface receptor TIM3. Both SB525334 and combined medication can reduce the expression of the NKG2A receptor on the surface of NK cells, but pirfenidone cannot. (G) Proliferative index Ki67of NK cells was an evident increase in the combined drug group compared to both the individual drugs and control groups. Data are representative of one of three independent experiments (n = 3–4 mice per group). One-way ANOVA was used for analysis in (B)–(F), bars represent the mean ± s.e.m. (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001).
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    Combination of pirfenidone and SB525334 altered the expression of NK cells’ receptors in mice with CE. (A) Mice were inoculated with 5000 protoscoleces, and pirfenidone and SB525334 were administered one month after infection. Samples were collected after one month of medication. (B) Only the pirfenidone and combination medication groups showing decreased secretion of <t>TGF-β</t> in CE liver tissues. (C) and (D) Compared to the other groups, serum ALT and AST levels are the lowest in the combined medication category. Similar results are evident with the levels of serum DBL. (E) Only SB525334 can reduce the secretion of DBL in the plasma of CE mice. (F) The three treatments elevate the expression of NK cell surface receptors DX5 and NKG2D and reduce the expression of NK cell surface receptor TIM3. Both SB525334 and combined medication can reduce the expression of the NKG2A receptor on the surface of NK cells, but pirfenidone cannot. (G) Proliferative index Ki67of NK cells was an evident increase in the combined drug group compared to both the individual drugs and control groups. Data are representative of one of three independent experiments (n = 3–4 mice per group). One-way ANOVA was used for analysis in (B)–(F), bars represent the mean ± s.e.m. (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001).
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    Image Search Results


    Journal: Cell reports

    Article Title: TGF-β in the microenvironment induces a physiologically occurring immune-suppressive senescent state

    doi: 10.1016/j.celrep.2023.112129

    Figure Lengend Snippet:

    Article Snippet: Rabbit polyclonal anti-TGF-β Receptor II , Cell Signaling Technology , Cat#79424; RRID:AB_2799933.

    Techniques: Virus, Sequencing, Recombinant, Staining, Enzyme-linked Immunosorbent Assay, DNA Library Preparation, Transfection, SYBR Green Assay, Viability Assay, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Derivative Assay, Real-time Polymerase Chain Reaction, Software

    Combination of pirfenidone and SB525334 altered the expression of NK cells’ receptors in mice with CE. (A) Mice were inoculated with 5000 protoscoleces, and pirfenidone and SB525334 were administered one month after infection. Samples were collected after one month of medication. (B) Only the pirfenidone and combination medication groups showing decreased secretion of TGF-β in CE liver tissues. (C) and (D) Compared to the other groups, serum ALT and AST levels are the lowest in the combined medication category. Similar results are evident with the levels of serum DBL. (E) Only SB525334 can reduce the secretion of DBL in the plasma of CE mice. (F) The three treatments elevate the expression of NK cell surface receptors DX5 and NKG2D and reduce the expression of NK cell surface receptor TIM3. Both SB525334 and combined medication can reduce the expression of the NKG2A receptor on the surface of NK cells, but pirfenidone cannot. (G) Proliferative index Ki67of NK cells was an evident increase in the combined drug group compared to both the individual drugs and control groups. Data are representative of one of three independent experiments (n = 3–4 mice per group). One-way ANOVA was used for analysis in (B)–(F), bars represent the mean ± s.e.m. (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001).

    Journal: Parasitology

    Article Title: A combination of pirfenidone and TGF-β inhibition mitigates cystic echinococcosis-associated hepatic injury

    doi: 10.1017/S0031182021000287

    Figure Lengend Snippet: Combination of pirfenidone and SB525334 altered the expression of NK cells’ receptors in mice with CE. (A) Mice were inoculated with 5000 protoscoleces, and pirfenidone and SB525334 were administered one month after infection. Samples were collected after one month of medication. (B) Only the pirfenidone and combination medication groups showing decreased secretion of TGF-β in CE liver tissues. (C) and (D) Compared to the other groups, serum ALT and AST levels are the lowest in the combined medication category. Similar results are evident with the levels of serum DBL. (E) Only SB525334 can reduce the secretion of DBL in the plasma of CE mice. (F) The three treatments elevate the expression of NK cell surface receptors DX5 and NKG2D and reduce the expression of NK cell surface receptor TIM3. Both SB525334 and combined medication can reduce the expression of the NKG2A receptor on the surface of NK cells, but pirfenidone cannot. (G) Proliferative index Ki67of NK cells was an evident increase in the combined drug group compared to both the individual drugs and control groups. Data are representative of one of three independent experiments (n = 3–4 mice per group). One-way ANOVA was used for analysis in (B)–(F), bars represent the mean ± s.e.m. (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001).

    Article Snippet: The following antibodies were used and diluted according to the manufactures’ recommendations: anti-p53 rabbit polyclonal antibody (# AF0879, affinity), anti-p21 mouse monoclonal antibody (#AP021, clone F-5, Beyotime), anti-CDKN2A/p16INK4a rabbit monoclonal antibody (#ab108349, clone:EPR1473, Abcam), anti-α smooth muscle actin rabbit monoclonal antibody (#ab124964, clone:EPR5368, Abcam), anti-TGF-β receptor I rabbit polyclonal antibody (#ab31013, Abcam), anti-TGF-β receptor II rabbit polyclonal antibody (#ab186838, Abcam), anti-collagen I rabbit monoclonal antibody (#ab6308, clone:COL-1, Abcam), anti-collagen III rabbit polyclonal antibody (#ab7778, Abcam), anti-MMP1 rabbit polyclonal antibody (#ab137332, Abcam), anti-TIMP1 monoclonal antibody (#MA1-773, clone:EPR5368, Thermo Fisher Scientific), goat anti-mouse IgG H&L (HRP) (#ab205719, Abcam), goat anti-mouse IgG H&L (HRP) (#ab205719, Abcam) and anti-β-actin mouse mAb (#AM4302, clone:AC-15, Cell Signalling Technology).

    Techniques: Expressing, Infection, Cell Surface Receptor Assay

    Increased secretion of IL-15 and TGF-β in the mouse liver with CE. (A) Secretion of IL-15 in serum at peak on the 30th day after infection and is maintained at high levels 90 days after infection in both the 3000 and 5000 protoscoleces groups, compared to the control. (B) The secretion of TGF-β in liver tissues at a maximum of 60 days after infection and is up-regulated within 90 days of infection in both the 3000 and 5000 protoscoleces groups, compared to the control. (C)–(F) Immunofluorescence showing that TGF-β1 mainly originated from macrophages in both mouse and human liver after developing CE. Note: Images for all the sections were taken using a microscope at 40× magnification. Scale bars, 50 μm. Data in (A) are representative of one of three independent experiments (n = 3–4 mice per group), bars represent the mean ± s.e.m.

    Journal: Parasitology

    Article Title: A combination of pirfenidone and TGF-β inhibition mitigates cystic echinococcosis-associated hepatic injury

    doi: 10.1017/S0031182021000287

    Figure Lengend Snippet: Increased secretion of IL-15 and TGF-β in the mouse liver with CE. (A) Secretion of IL-15 in serum at peak on the 30th day after infection and is maintained at high levels 90 days after infection in both the 3000 and 5000 protoscoleces groups, compared to the control. (B) The secretion of TGF-β in liver tissues at a maximum of 60 days after infection and is up-regulated within 90 days of infection in both the 3000 and 5000 protoscoleces groups, compared to the control. (C)–(F) Immunofluorescence showing that TGF-β1 mainly originated from macrophages in both mouse and human liver after developing CE. Note: Images for all the sections were taken using a microscope at 40× magnification. Scale bars, 50 μm. Data in (A) are representative of one of three independent experiments (n = 3–4 mice per group), bars represent the mean ± s.e.m.

    Article Snippet: The following antibodies were used and diluted according to the manufactures’ recommendations: anti-p53 rabbit polyclonal antibody (# AF0879, affinity), anti-p21 mouse monoclonal antibody (#AP021, clone F-5, Beyotime), anti-CDKN2A/p16INK4a rabbit monoclonal antibody (#ab108349, clone:EPR1473, Abcam), anti-α smooth muscle actin rabbit monoclonal antibody (#ab124964, clone:EPR5368, Abcam), anti-TGF-β receptor I rabbit polyclonal antibody (#ab31013, Abcam), anti-TGF-β receptor II rabbit polyclonal antibody (#ab186838, Abcam), anti-collagen I rabbit monoclonal antibody (#ab6308, clone:COL-1, Abcam), anti-collagen III rabbit polyclonal antibody (#ab7778, Abcam), anti-MMP1 rabbit polyclonal antibody (#ab137332, Abcam), anti-TIMP1 monoclonal antibody (#MA1-773, clone:EPR5368, Thermo Fisher Scientific), goat anti-mouse IgG H&L (HRP) (#ab205719, Abcam), goat anti-mouse IgG H&L (HRP) (#ab205719, Abcam) and anti-β-actin mouse mAb (#AM4302, clone:AC-15, Cell Signalling Technology).

    Techniques: Infection, Immunofluorescence, Microscopy

    The combination of pirfenidone and SB525334 could be relieving CE-related liver fibrosis. Using inhibitors of the TGF-β signalling pathway, immunoblot experiments highlight the expression of fibrosis-related proteins in the liver tissues of CE mice treated with different groups of drugs. Data are representative of one of three independent experiments (n = 3–4 mice per group). One-way ANOVA was used for analysis, bars represent the mean ± s.e.m. (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001).

    Journal: Parasitology

    Article Title: A combination of pirfenidone and TGF-β inhibition mitigates cystic echinococcosis-associated hepatic injury

    doi: 10.1017/S0031182021000287

    Figure Lengend Snippet: The combination of pirfenidone and SB525334 could be relieving CE-related liver fibrosis. Using inhibitors of the TGF-β signalling pathway, immunoblot experiments highlight the expression of fibrosis-related proteins in the liver tissues of CE mice treated with different groups of drugs. Data are representative of one of three independent experiments (n = 3–4 mice per group). One-way ANOVA was used for analysis, bars represent the mean ± s.e.m. (ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001).

    Article Snippet: The following antibodies were used and diluted according to the manufactures’ recommendations: anti-p53 rabbit polyclonal antibody (# AF0879, affinity), anti-p21 mouse monoclonal antibody (#AP021, clone F-5, Beyotime), anti-CDKN2A/p16INK4a rabbit monoclonal antibody (#ab108349, clone:EPR1473, Abcam), anti-α smooth muscle actin rabbit monoclonal antibody (#ab124964, clone:EPR5368, Abcam), anti-TGF-β receptor I rabbit polyclonal antibody (#ab31013, Abcam), anti-TGF-β receptor II rabbit polyclonal antibody (#ab186838, Abcam), anti-collagen I rabbit monoclonal antibody (#ab6308, clone:COL-1, Abcam), anti-collagen III rabbit polyclonal antibody (#ab7778, Abcam), anti-MMP1 rabbit polyclonal antibody (#ab137332, Abcam), anti-TIMP1 monoclonal antibody (#MA1-773, clone:EPR5368, Thermo Fisher Scientific), goat anti-mouse IgG H&L (HRP) (#ab205719, Abcam), goat anti-mouse IgG H&L (HRP) (#ab205719, Abcam) and anti-β-actin mouse mAb (#AM4302, clone:AC-15, Cell Signalling Technology).

    Techniques: Western Blot, Expressing